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Effect of Air Plasma Processing on the Adsorption Behaviour of Bovine Serum Albumin on Spin-Coated PMMA Surfaces

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Liu, C and Meenan, BJ (2008) Effect of Air Plasma Processing on the Adsorption Behaviour of Bovine Serum Albumin on Spin-Coated PMMA Surfaces. Journal of Bionic Engineering, 5 (3). pp. 204-214. [Journal article]

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URL: http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B82XN-4TP37BC-5&_user=126978&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_searchStrId=1163482628&_rerunOrigin=google&_acct=C000010438&_version=1&_urlVersion=0&_userid=126978&md5=6bd0ed9fc3

DOI: doi:10.1016/S1672-6529(08)60026-8

Abstract

This paper reports the adsorption of Bovine Serum Albumin (BSA) onto Dielectric Barrier Discharge (DBD) processed Poly(methyl methacrylate) (PMMA) surfaces by a Quartz Crystal Microbalance with Dissipation monitoring (QCM-D) technique. The purpose is to study the influence of DBD processing on the nature and scale of BSA adsorption on PMMA surface in vitro. It was observed that DBD processing improves the surface wettability of PMMA film, a fact attributable to the changes in surface chemistry and topography. Exposure of the PMMA to Phosphate Buffed Saline (PBS) solution in the QCM-D systemresulted in surface adsorption which reaches an equilibrium after about 30 minutes for pristine PMMA, and 90 minutes for processed PMMA surface. Subsequent injection of BSA in PBS indicated that the protein is immediately adsorbed onto the PMMA surface. It was revealed that adsorption behaviour of BSA on pristine PMMA differs from that on processed PMMA surface. A slower adsorption kinetics was observed for pristine PMMA surface, whilst a quick adsorption kinetics for processed PMMA. Moreover, the dissipation shift of protein adsorption suggested that BSA forms a more rigid structure on pristine PMMA surface that on processed surface. These data suggest that changes in wettability and attendant chemical properties and surface texture of the PMMA surface may play a significant role in BSA adsorption process.Keywords: in vitro test, protein adsorption, surface modification, polymethylmethacrylate, QCM-DCopyright © 2008, Jilin University. Published by Elsevier Limited and Science Press. All rights reserved.

Item Type:Journal article
Faculties and Schools:Faculty of Computing & Engineering
Faculty of Computing & Engineering > School of Engineering
Research Institutes and Groups:Engineering Research Institute
Engineering Research Institute > Nanotechnology & Integrated BioEngineering Centre (NIBEC)
ID Code:6886
Deposited By:Professor Brian Meenan
Deposited On:20 Jan 2010 09:36
Last Modified:18 Aug 2011 11:53

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