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THE ISOLATION AND CHARACTERIZATION OF A SALICYLATE-HYDROXYLASE-PRODUCING STRAIN OF PSEUDOMONAS-PUTIDA

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Banat, IM, Singh - Nee Nigam, Poonam and Marchant, R (1992) THE ISOLATION AND CHARACTERIZATION OF A SALICYLATE-HYDROXYLASE-PRODUCING STRAIN OF PSEUDOMONAS-PUTIDA. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 37 (3). pp. 378-381. [Journal article]

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Abstract

A salicylate-hydroxylase-producing strain of Pseudomonas putida with an unusual capability to grow at toxic levels of salicylate up to 10 g l-1 has been isolated. It grew well under continuous culture conditions, with optimum growth at pH 6.5 and a temperature of 25-degrees-C. The use of an ammonium salt as a nitrogen source, instead of nitrate, resulted in a 30-40% increase in its biomass yield coefficient. Optimum growth under continuous culture conditions was achieved using 4 g l-1 salicylate at 25-degrees-C, pH 6.5 and 0.2 h-1 dilution rate. High salicylate hydroxylase enzyme activity [236 units (U) l-1] and productivity (424.8 U h-1) were obtained at a dilution rate of 0.45 h-1 using a mineral medium containing 4 g l-1 of salicylate. Operating under continuous culture conditions with oxygen limitation and a slight accumulation of residual salicylate (0.2 g l-1) resulted in a decrease in culture performance and enzyme productivity.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Infection and Immunity/Microbiology
Biomedical Sciences Research Institute > Pharmaceutical Science and Practice
ID Code:5574
Deposited By:Dr Poonam Singh - Nee Nigam
Deposited On:21 Dec 2009 11:58
Last Modified:16 May 2012 14:30

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