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PRODUCTION OF THE ENZYME DIHYDROFOLATE-REDUCTASE BY METHOTREXATE-RESISTANT BACTERIA ISOLATED FROM SOIL

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Singh - Nee Nigam, Poonam and Marchant, R (1993) PRODUCTION OF THE ENZYME DIHYDROFOLATE-REDUCTASE BY METHOTREXATE-RESISTANT BACTERIA ISOLATED FROM SOIL. JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, 56 (1). pp. 35-40. [Journal article]

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Abstract

Six bacterial cultures isolated from soil were capable of growing in the presence of methotrexate (MTX). Two strains, PFR-1 and 3, developed resistance to 500 mug cm-3 MTX in the medium and produced elevated levels of the enzyme dihydrofolate reductase (EC 1. 5.1. 3): 2580 and 2702 U dm-3 compared to the sensitive parent strains (28 and 35 U dm-3). Isolate PFR-3 showed maximum enzyme production (4950 U dm-3, specific activity 12.56 U mg-1 in flasks and 5737 U dm-3, specific activity 14.80 U mg-1 in 5-dm3 fermenter) during exponential phase of growth (6 h) at 37-degrees-C and pH 7.0.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Infection and Immunity/Microbiology
ID Code:5572
Deposited By:Dr Poonam Singh - Nee Nigam
Deposited On:21 Dec 2009 11:57
Last Modified:04 Aug 2011 09:48

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