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PRODUCTION OF SALICYLATE HYDROXYLASE FROM PSEUDOMONAS-PUTIDA UUC-1 AND ITS APPLICATION IN THE CONSTRUCTION OF A BIOSENSOR

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Zhou, DM, Singh - Nee Nigam, Poonam, Jones, J and Marchant, Roger (1995) PRODUCTION OF SALICYLATE HYDROXYLASE FROM PSEUDOMONAS-PUTIDA UUC-1 AND ITS APPLICATION IN THE CONSTRUCTION OF A BIOSENSOR. JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, 64 (4). pp. 331-338. [Journal article]

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Abstract

The enzyme salicylate hydroxylase was produced from Pseudomonas putida UUC-1 in an 80 dm(3) 16 h batch fermentation process yielding, similar to 9 K units per 60 dm(3). This enzyme preparation has been studied for its application in the construction of biosensor systems, e.g. carbon paste electrodes, screen-printed carbon electrodes, and disposable carbon enzyme electrodes, which will be used for the rapid estimation of salicylate in blood samples. The linear range of the disposable carbon enzyme electrode in response to salicylate was achieved to 1.8 mmol dm(-3).

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Infection and Immunity/Microbiology
ID Code:5559
Deposited By:Dr Poonam Singh - Nee Nigam
Deposited On:21 Dec 2009 11:33
Last Modified:27 Jun 2011 13:37

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