Ulster University Logo

Ulster Institutional Repository

Thermostable, alkalophilic and cellulase free xylanase production by Thermoactinomyces thalophilus subgroup C

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Kohli, U, Singh - Nee Nigam, Poonam, Singh, D and Chaudhary, K (2001) Thermostable, alkalophilic and cellulase free xylanase production by Thermoactinomyces thalophilus subgroup C. ENZYME AND MICROBIAL TECHNOLOGY, 28 (7-8). pp. 606-610. [Journal article]

Full text not available from this repository.

Abstract

Thermoactinomyces thalophilus produced cellulase free extracellular endo-1,4-beta -xylanase (EC 3.2.1.8) at 50 degreesC and pH 8.5. Maximum xylanase production was thieved in fermentation medium using birchwood xylan as substrate after 96 h of growth at 50 degreesC. Other agricultural substrates such as wheat bran, wheat straw, sugarcane bagnsse and cornstover produced less xylanase, The crude enzyme preparation from mutant T. thalophilus P2 grown under optimised fermentation conditions showed no cellulase contamination and maximum xylanase activity of 42 U/ml at 65 degreesC and pH 8.5-9.0. This enzyme with initial sylanase activity of 42 U/ml was found thermostable up to 65 degreesC and retaining 50% of its activity after its incubation for 125 min at 65 degreesC. (C) 2001 Elsevier Science Inc. All rights reserved.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Infection and Immunity/Microbiology
ID Code:5495
Deposited By:Dr Poonam Singh - Nee Nigam
Deposited On:21 Dec 2009 10:54
Last Modified:04 Aug 2011 09:48

Repository Staff Only: item control page