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A response surface approach for the comparison of lipase production by Candida cylindracea using two different carbon sources

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Muralidhar, RV, Chirumamila, RR, Marchant, R and Singh - Nee Nigam, Poonam (2001) A response surface approach for the comparison of lipase production by Candida cylindracea using two different carbon sources. BIOCHEMICAL ENGINEERING JOURNAL, 9 (1). pp. 17-23. [Journal article]

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Abstract

A response surface approach has been used to study the production of extracellular lipase by Candida cylindracea. Medium constituents were optimised for the production of lipase by central composite design. A 2(5) design matrix was used in the experiments with only the carbon source being varied. The optimal yield of lipase was 17.30 U/ml using glucose as the carbon source and 47.25 U/ml using olive oil as the carbon source. All the other medium constituents namely yeast extract, malt extract, peptone and tween 80 were simultaneously optimised. Experiments conducted at the predicted optimum levels fitted with the predicted optima. Based on these experimental conditions, medium containing olive oil appears to a better medium for fermentative production of C. cylindracea lipase. (C) 2001 Elsevier Science B.V. All rights reserved.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Infection and Immunity/Microbiology
ID Code:5488
Deposited By:Dr Poonam Singh - Nee Nigam
Deposited On:21 Dec 2009 10:48
Last Modified:04 Aug 2011 09:48

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