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BIOACTIVITY OF EXTRACTS OF CENTAUREA POLYCLADA DC. (ASTERACEAE)

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Granger, Melanie, Samson, Emilie, Sauvage, Severine, Majumdar, Anisha, Singh - Nee Nigam, Poonam, Nahar, Lutfun, Celik, Sezgin and Sarker, Satyajit D. (2009) BIOACTIVITY OF EXTRACTS OF CENTAUREA POLYCLADA DC. (ASTERACEAE). ARCHIVES OF BIOLOGICAL SCIENCES, 61 (3). pp. 447-452. [Journal article]

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DOI: 10.2298/ABS0903447G

Abstract

The free radical-scavenging property, antibacterial activity, and brine shrimp toxicity of n-hexane, dichloromethane (DCM), and methanol (MeOH) extracts of Centaurea polyclada, an endemic Turkish species, were assessed using the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assay, the resazurin microtiter plate-based assay, and the brine shrimp lethality assay, respectively. The DCM and MeOH extracts of C. polyclada exhibited free radical-scavenging ability with RC50 values 1.17 and 0.015 mg/mL, respectively. Among solid-phase extraction fractions of the MeOH extract, the fraction eluted with 60% MeOH in water demonstrated the highest level of free radical-scavenging activity (RC50 = 0.016 mg/mL). Only the DCM extract showed considerable antibacterial activity against all nine test strains except Escherichia coli, with MIC ranging from 1.25 to 2.50 mg/mL. This antibacterial activity pattern was also observed with solid-phase extraction fractions of the DCM extract with varied potencies. None of the extracts showed any significant toxicity towards brine shrimps (LD50 = >1.00 mg/mL).

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Infection and Immunity/Microbiology
ID Code:5448
Deposited By:Dr Poonam Singh - Nee Nigam
Deposited On:21 Dec 2009 10:11
Last Modified:04 Aug 2011 09:47

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