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Effect of antioxidant vitamin supplementation on DNA damage and repair in human lymphoblastoid cells

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Sweetman, SF, Strain, JJ and McKelvey-Martin, VJ (1997) Effect of antioxidant vitamin supplementation on DNA damage and repair in human lymphoblastoid cells. NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL, 27 (2). pp. 122-130. [Journal article]

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Abstract

In this study the possible protective effects of ascorbic acid and alpha-tocopherol (singly and in combination) on Raji lymphoblastoid cells exposed to various doses of X-rays or hydrogen peroxide (H2O2) are investigated. DNA strand breaks and alkali-labile sites were measured using the alkaline comet assay. Survival and hypoxanthine guanine phosphoribosyl transferase mutant frequency were measured using the colony-forming assay. Ascorbic acid (60 mu M) and alpha-tocapherol (30 mu M) were added singly or together to cell culture medium 24 hours before treatment and were present during treatment. After the 24-hour supplementation period with ascorbic acid atone, alpha-tocopherol alone, and ascorbic acid + alpha-tocopherol, the level of endogenous DNA damage was significantly lower (p < 0.05) than in the nonsupplemented culture, as assessed by the comet assay. By use of the comet assay, it was observed that ascorbic acid exhibited an overall protective effect against DNA damage induced after X-ray treatment, whereas alpha-tocopherol exhibited an overall protective effect against DNA damage induced after H2O2 treatment. Significant increases were observed in the percent survival after 1-Gy X-rays and 5 and 20 mu M H2O2 in those cultures supplemented with ascorbic acid alone and alpha-tocopherol alone relative to the nonsupplemented cultures. The endogenous level of mutant frequency was also significantly decreased in the presence of ascorbic acid relative to the nonsupplemented culture. These findings are consistent with the concept that ascorbic acid and alpha-tocopherol can, under certain conditions, protect against oxidatively induced DNA damage.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Faculty of Life and Health Sciences > School of Pharmacy and Pharmaceutical Science
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Northern Ireland Centre for Food and Health (NICHE)
ID Code:5271
Deposited By:Mrs Alison Deehan
Deposited On:14 Jan 2010 15:01
Last Modified:04 Dec 2012 12:31

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