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Simultaneous automated measurement of total `antioxidant' (reducing) capacity and ascorbic acid concentration

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Benzie, IFF and Strain, JJ (1997) Simultaneous automated measurement of total `antioxidant' (reducing) capacity and ascorbic acid concentration. Redox Report, 3 (4). pp. 233-238. [Journal article]

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Abstract

Biochemical tests of antioxidant status may be useful in measuring oxidative stress, however the time, skills and resources required for most such tests have limited their application and evaluation. In this study, newly available tests for two indices of antioxidant status, ferric reducing ability of plasma (FRAP) and ascorbic acid, were combined in one simple, rapid, automated test, referred to as FRASC. Results are available within 5 min of sample/reagent mixing. Linear dose-response is seen over a wide concentration range for both parameters. In- and between-run CVs were <1.0% and <3.0% respectively at 900-1800 mu mol/l FRAP, and <5.0% for both at 20-400 mu mol/l ascorbic acid. Mean (SD) FRAP values and ascorbic acid concentrations of fasting plasma from 130 healthy adults were 1018 (198) and 51 (18) mu mol/l respectively. This combined test is straightforward and inexpensive, and lends itself to the measurement of `antioxidant' (reducing) capacity and ascorbic acid concentrations of various biological fluids, as well as extracts of drugs and foodstuffs. FRASC will, therefore, facilitate experimental and clinical studies of pro-oxidant: antioxidant balance and oxidative stress-related disease.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Northern Ireland Centre for Food and Health (NICHE)
ID Code:5265
Deposited By:Mrs Alison Deehan
Deposited On:14 Jan 2010 14:59
Last Modified:22 Aug 2012 10:39

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