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The effect of copper supplementation on red blood cell oxidizability and plasma antioxidants in middle-aged healthy volunteers

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Rock, E, Mazur, A, O'Connor, JM, Bonham, MP, Rayssiguier, Y and Strain, JJ (2000) The effect of copper supplementation on red blood cell oxidizability and plasma antioxidants in middle-aged healthy volunteers. FREE RADICAL BIOLOGY AND MEDICINE, 28 (3). pp. 324-329. [Journal article]

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A multicenter European study (FoodCue) was undertaken to provide data on the significance of increased dietary copper as a pro-oxidant or antioxidant in vivo. The present work describes the effect of Cu supplementation on (2,2' -azo-bis(2-amidinopropane) hydrochloride (AAPH)-induced red blood cell oxidation in middle-aged people. Double-blinded copper supplementation was achieved in 26 healthy volunteers (50-70 years) with pills containing 3 mg CuSO4, 3 mg Cu glycine chelate (CuG) and 6 mg Cue. Each 6 week supplementation period was preceded and followed by 6 weeks of washout (WO) on placebo. The results show significant increases in time necessary to achieve 50% hemolysis (LT50) after 3CuSO(4) and 6CuG compared with values after WO periods. Cu supplementation did not increase the levels of (Cu,Zn)SOD activity in red blood cells. Resistance to hemolysis was significantly and positively correlated (r = .30, p < .01) with alpha- and beta-carotene content in the plasma. Together,these data suggest that intake of copper as high as 7 mg/d has no pro-oxidant activity and may rather result in protection of red blood cells against oxidation. The decreased oxidizability of red blood cells did not result from increased (Cu,Zn)SOD activity and may occur through other mechanisms such as changes in membrane antioxidant content. (C) 2000 Elsevier Science Inc.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Northern Ireland Centre for Food and Health (NICHE)
ID Code:5239
Deposited By:Mrs Alison Deehan
Deposited On:14 Jan 2010 14:47
Last Modified:15 Dec 2011 11:53

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