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Peptide Tyrosine Arginine, a potent immunomodulatory peptide isolated and structurally characterized from the skin secretions of the dusky gopher frog, Rana sevosa

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Graham, C, Irvine, A. E., McClean, Stephen, Richter, S. C., Flatt, Peter and Shaw, C. (2005) Peptide Tyrosine Arginine, a potent immunomodulatory peptide isolated and structurally characterized from the skin secretions of the dusky gopher frog, Rana sevosa. Peptides, 26 (5). pp. 737-743. [Journal article]

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Abstract

An octadecapeptide was isolated from the skin secretions of the dusky gopher frog (Rana sevosa) on the basis of histamine release from rat peritoneal mast cells. This peptide was purified to homogeneity by HPLC and found to have the following primary structure, YLKGCWTKSYPPKPCFSR, using both Edman degradation chemistry and peptide sequencing using high-resolution mass spectrometry (Q-TOF MS). The peptide, named peptide Tyrosine Arginine (pYR) shares 77.8% homology with peptide Leucine Arginine (pLR). The effects of the natural amidated peptide, non-amidated peptide and C-loop region of pYR on granulopoiesis and neutrophil apoptosis were investigated. All three analogues inhibited the early development of granulocyte macrophage colonies from bone marrow stem cells but did not induce apoptosis of the end stage granulocytes, the mature neutrophil. Thus, pYR is a novel member of an important and emerging new class of amphibian peptides with hemopoietic actions.

Item Type:Journal article
Keywords:Amino Acid Sequence; Amphibian Proteins/chemistry/isolation & purification/pharmacology; Animals; Colony-Forming Units Assay; Granulocytes/drug effects; Histamine Release/drug effects; Immunologic Factors/chemistry/isolation & purification/pharmacology; Macrophages/drug effects; Male; Mast Cells/drug effects; Molecular Sequence Data; Peptides/chemistry/isolation & purification; Ranidae/metabolism; Rats; Skin/secretion
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Diabetes
Biomedical Sciences Research Institute > Pharmaceutical Science and Practice
ID Code:5066
Deposited By:Dr Stephen McClean
Deposited On:04 Feb 2010 09:39
Last Modified:26 Jul 2011 15:48

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