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NUTRITIONAL-REQUIREMENTS AND GROWTH-CHARACTERISTICS OF A BIOSURFACTANT - PRODUCING RHODOCOCCUS BACTERIUM

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Aburuwaida, AS, Banat, Ibrahim, Haditirto, S and Khamis, A (1991) NUTRITIONAL-REQUIREMENTS AND GROWTH-CHARACTERISTICS OF A BIOSURFACTANT - PRODUCING RHODOCOCCUS BACTERIUM. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 7 (1). pp. 53-61. [Journal article]

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Abstract

The nutritional requirements and growth characteristics of a biosurfactant-producing Rhodococcus bacterium isolated from Kuwaiti soil were determined. Maximum cell yields (6.6 g/l) and biosurfactant production were achieved with a medium containing 2% (v/v) n-paraffin as a carbon and energy source, 0.2% lactose broth, optimal concentrations of nitrogen (nitrate), phosphorus, iron, magnesium and sodium sources, and minimal concentrations of potassium and trace element sources. The optimal pH was 6.8 for surfactant production and optimal temperature was 37-degrees-C. The biosurfactant produced after 16 to 33 h growth in a 7 I fermenter decreased both surface tension and interfacial tension of culture broth to below 27 and 1.8 mN/m, respectively, and was effective at critical micelle dilutions of 10(-3). Data on biosurfactant biosynthesis suggest that the product is produced as a primary metabolite and, therefore, could be produced effectively under continuous fermentation conditions.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Pharmaceutical Science and Practice
ID Code:4355
Deposited By:Professor Ibrahim Banat
Deposited On:06 Jan 2010 16:43
Last Modified:16 May 2012 14:32

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