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High alcohol production by repeated batch fermentation using an immobilized osmotolerant Saccharomyces cerevisiae

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Sree, NK, Sridhar, M, Suresh, K, Banat, Ibrahim and Rao, LV (2000) High alcohol production by repeated batch fermentation using an immobilized osmotolerant Saccharomyces cerevisiae. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 24 (3). pp. 222-226. [Journal article]

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Abstract

A repeated batch fermentation system was used to produce ethanol using an osmotolerant Saccharomyces cerevisiae (VS3) immobilized in calcium alginate beads, For comparison free cells were also used to produce ethanol by repeated batch fermentation. Fermentation was carried for six cycles with 125, 250 or 500 beads using 150, 200 or 250 g glucose L-1 at 30 degrees C, The maximum amount of ethanol produced by immobilized VS, using 150 g L-1 glucose was only 44 g L-1 after 48 h, while the amount of ethanol produced by free cells in the first cycle was 72 g L-1. However in subsequent fed batch cultures more ethanol was produced by immobilized cells compared to free cells. The amount of ethanol produced by free cells decreased from 72 g L-1 to 25 g L-1 after the fourth cycle, while that of immobilized cells increased from 44 to 72 g L-1, The maximum amount of ethanol produced by immobilized VS, cells using 150, 200 and 250 g glucose L-1 was 72.5, 93 and 87 g ethanol L-1 at 30 degrees C.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Pharmaceutical Science and Practice
ID Code:4288
Deposited By:Professor Ibrahim Banat
Deposited On:07 Jan 2010 20:22
Last Modified:16 May 2012 12:33

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