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The Use of Conjunctival Swab for the Proteomic Characterisation of Dry Eye Syndrome

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Graham, JE, Graham, RLJ, Beirne, Raymond, McGilligan, Victoria, Downes, Stephen, Moore, Johnny, Moore, Tara and McMullan, Geoffrey (2008) The Use of Conjunctival Swab for the Proteomic Characterisation of Dry Eye Syndrome. Journal of Proteomics and Bioinformatics, 1 . pp. 20-33. [Journal article]

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URL: http://www.omicsonline.com/Archive/HTMLApril2008/JPB1.17.html

Abstract

In this study we report the first gel based proteomic analysis of an inflammed dry eye utilising a clinically-based non-invasive methodology for collection of a specimen from the posterior lid and inferior conjunctival mucosa of the subject. This multidimensional technique allowed the identification of 592 proteins, having a MOWSE score of greater than 40, using the heuristic tool PROVALT. Automated curation of this list using an inbuilt randomised database searching tool with false discovery rate set at 1% significantly reduced this list to 86 proteins. Additional manual curation resulted in the final positive identification of 75 proteins. These identified proteins were functionally classified and physiochemically characterised. This led to the identification of a number of proteins involved in cell structure, inflammation, and the innate immune response. Contained within these proteins were a number of potential biomarkers of not only dry eye syndrome but also lacrimal gland acinar cell function such as lacritin, calgranulin A and lacrimal proline-rich protein 4.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Molecular Medicine
Biomedical Sciences Research Institute > Northern Ireland Centre for Food and Health (NICHE)
Biomedical Sciences Research Institute > Stratified Medicine
Biomedical Sciences Research Institute > Vision
Biomedical Sciences Research Institute > Molecular Medicine > Nano Systems Biology
ID Code:4147
Deposited By:Professor Tara Moore
Deposited On:08 Jan 2010 11:22
Last Modified:20 Feb 2014 11:44

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