Graham, JE, Graham, RLJ, Beirne, Raymond, McGilligan, Victoria, Downes, Stephen, Moore, JE, Moore, Tara and McMullan, Geoffrey (2008) The Use of Conjunctival Swab for the Proteomic Characterisation of Dry Eye Syndrome. Journal of Proteomics and Bioinformatics, 1 . pp. 20-33. [Journal article]
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In this study we report the first gel based proteomic analysis of an inflammed dry eye utilising a clinically-based non-invasive methodology for collection of a specimen from the posterior lid and inferior conjunctival mucosa of the subject. This multidimensional technique allowed the identification of 592 proteins, having a MOWSE score of greater than 40, using the heuristic tool PROVALT. Automated curation of this list using an inbuilt randomised database searching tool with false discovery rate set at 1% significantly reduced this list to 86 proteins. Additional manual curation resulted in the final positive identification of 75 proteins. These identified proteins were functionally classified and physiochemically characterised. This led to the identification of a number of proteins involved in cell structure, inflammation, and the innate immune response. Contained within these proteins were a number of potential biomarkers of not only dry eye syndrome but also lacrimal gland acinar cell function such as lacritin, calgranulin A and lacrimal proline-rich protein 4.
|Item Type:||Journal article|
|Faculties and Schools:||Faculty of Life and Health Sciences|
Faculty of Life and Health Sciences > School of Biomedical Sciences
|Research Institutes and Groups:||Biomedical Sciences Research Institute|
Biomedical Sciences Research Institute > Infection and Immunity/Microbiology
Biomedical Sciences Research Institute > Molecular Medicine
Biomedical Sciences Research Institute > Northern Ireland Centre for Food and Health (NICHE)
Biomedical Sciences Research Institute > Vision
Biomedical Sciences Research Institute > Molecular Medicine > Nano Systems Biology
|Deposited By:||Professor Tara Moore|
|Deposited On:||08 Jan 2010 11:22|
|Last Modified:||16 Jan 2013 11:49|
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