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Characterization of rhamnolipids produced by a Pseudomonas aeruginosa mutant strain grown on waste oils

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Raza, Zulfiqar A., Khalid, Zafar M. and Banat, Ibrahim (2009) Characterization of rhamnolipids produced by a Pseudomonas aeruginosa mutant strain grown on waste oils. JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART A-TOXIC/HAZARDOUS SUBSTANCES & ENVIRONMENTAL ENGINEERING, 44 (13). pp. 1367-1373. [Journal article]

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DOI: 10.1080/10934520903217138

Abstract

Pseudomonas aeruginosa EBN-8 mutant rhamnolipids produced on waste oils were investigated using normal-phase thin layer chromatography and fast atom bombardment mass spectrometry. Negative ion mode mass spectra yielded [M - H](-) ions and their fragment ions, which gave some indications on the sequence of rhamnolipid biosynthesis. Five rhamnolipid homologs [viz. RC10C10 (m/z 503), RC12C10 or RC10C12 (531), RRC10C8 or RRC8C10 (621), RRC10C10 (649) and RRC12C10 or RRC10C12 (677)] were detected in four rhamnolipid combinations under the different carbon sources. The prevalence of rhamnolipids was confirmed by Fourier transform infrared and one-dimensional proton nuclear magnetic resonance. We also observed some correlations between the tensioactive characteristics and structural chemistry of the rhamnolipid surfactants.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Pharmaceutical Science and Practice
ID Code:4119
Deposited By:Professor Ibrahim Banat
Deposited On:14 Jan 2010 10:59
Last Modified:16 May 2012 11:12

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