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The Expression of Membrane-Associated 67-kDa Laminin Receptor (67LR) Is Modulated in Vitro by Cell-Contact Inhibition

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Donaldson, EA, McKenna, Declan J, Moore, Tara, Scott, WN, Stitt, AW and Nelson, J (2000) The Expression of Membrane-Associated 67-kDa Laminin Receptor (67LR) Is Modulated in Vitro by Cell-Contact Inhibition. Molecular Cell Biology Research Communications, 3 . [Journal article]

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DOI: doi:10.1006/mcbr.2000.0191

Abstract

The interaction of cells with their substratum is an important determinant of cell behaviour, influencing attachment, proliferation, and motility. Such interactions are mediated by cell surface receptors which bind to attachment factors, like the glycoprotein laminin in basement membranes. We have previously shown that expression of the 67-kDa laminin receptor (67LR) is elevated in proliferating retinal microvasculature compared with mature, quiescent vessels. Here, we examined 67LR mRNA and protein expression in primary cultures of retinal microvascular endothelial cells (RMEC) and in the breast cancer cell-line T47D during stages of contact inhibition. In both cell types, the expression levels of 67LR mRNA and membrane-associated 67LR protein were significantly increased during the proliferative phases of monolayer formation. As the cells achieved contact inhibition, 67LR expression was reduced to comparatively low levels. Thus, the differential expression of 67LR between dividing and contact-inhibited cells may indicate a role for this receptor during proliferative processes.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Molecular Medicine
Biomedical Sciences Research Institute > Vision
Biomedical Sciences Research Institute > Molecular Medicine > Transcriptional Regulation & Epigenetics
ID Code:4103
Deposited By:Professor Tara Moore
Deposited On:18 Dec 2009 14:53
Last Modified:16 Jan 2013 12:38

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