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In vivo macular pigment measurements: a comparison of resonance Raman spectroscopy and heterochromatic flicker photometry

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Hogg, R. E., Anderson, Roger, Stevenson, M. R., Zlatkova, Margarita and Chakravarthy, U. (2007) In vivo macular pigment measurements: a comparison of resonance Raman spectroscopy and heterochromatic flicker photometry. BRITISH JOURNAL OF OPHTHALMOLOGY, 91 (4). pp. 485-490. [Journal article]

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DOI: 10.1136/bjo.2006.090936


Aim: To investigate whether two methods of measuring macular pigment-namely, heterochromatic flicker photometry (HFP) and resonance Raman spectroscopy (RRS)-yield comparable data. Methods: Macular pigment was measured using HFP and RRS in the right eye of 107 participants aged 20 79 years. Correlations between methods were sought and regression models generated. RRS was recorded as Raman counts and HFP as macular pigment optical density (MPOD). The average of the top three of five Raman counts was compared with MPOD obtained at 0.5 eccentricity, and an integrated measure (spatial profile; MPODsp) computed from four stimulus sizes on HFP. Results: The coefficient of variation was 12.0% for MPODsp and 13.5% for Raman counts. MPODsp exhibited significant correlations with Raman counts (r=0.260, p=0.012), whereas MPOD at 0.5 did not correlate significantly (r=0.163, p=0.118). MPODsp was not significantly correlated with age (p=0.062), whereas MPOD at 0.5 was positively correlated (p=0.011). Raman counts showed a significant decrease with age (p=0.002) and were significantly lower when pupil size was smaller (p=0.015). Conclusions: Despite a statistically significant correlation, the correlations were weak, with those in excess of 90% of the variance between MPODsp and Raman counts remaining unexplained, meriting further research.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Vision
ID Code:3929
Deposited By:Dr Margarita Vidinova
Deposited On:09 Feb 2010 11:49
Last Modified:04 Aug 2011 09:51

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