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Molecular epidemiology of clinical isolates of Pseudomonas aeruginosa isolated from horses in Ireland

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Tazumi, A., Maeda, Y., Buckley, T., Millar, B. C., Goldsmith, C. E., Dooley, James, Elborn, J. S., Matsuda, M. and Moore, J. E. (2009) Molecular epidemiology of clinical isolates of Pseudomonas aeruginosa isolated from horses in Ireland. IRISH VETERINARY JOURNAL, 62 (7). pp. 456-459. [Journal article]

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Abstract

Clinical isolates (n=63) of Pseudomonas aeruginosa obtained from various sites in 63 horses were compared using ERIC2 RAPD PCR to determine their genetic relatedness. Resulting banding patterns (n=24 genotypes) showed a high degree of genetic heterogeneity amongst all isolates examined, indicating a relative non-clonal relationship between isolates from these patients, employing this genotyping technique. This study characterised 63 clinical isolates into 24 distinct genotypes, with the largest cluster (genotype E) accounting for 10/63 (15.9%) of the isolates. ERIC2 RAPID PCR proved to be a highly discriminatory molecular typing tool of P. aeruginosa in isolates recovered from horses. With the adoption of several controls to aid reproducibility, this technique may be useful as an alternative to PFGE, particularly in epidemiological investigations of outbreaks where speed may be a significant parameter. This is the first report of clonal heterogeneity amongst P. aeruginosa from horses and demonstrated that ERIC RAPID PCR is a rapid method for the examination of this species in horses, which may be useful in outbreak analysis.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Northern Ireland Centre for Food and Health (NICHE)
ID Code:3798
Deposited By:Professor James Dooley
Deposited On:21 Dec 2009 16:43
Last Modified:17 May 2012 11:10

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