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Estimating leukocyte velocities from high-speed 1D line scans oriented orthogonal to blood flow

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Bigot, S., Lucas, L., Morrow, PJ, Mitchell, Christopher and Saetzler, Kurt (2007) Estimating leukocyte velocities from high-speed 1D line scans oriented orthogonal to blood flow. In: Biomedical Imaging: Macro to Nano, 2007. 4rd IEEE International Symposium on, Washington. UNSPECIFIED. 4 pp. [Conference contribution]

Full text not available from this repository.

DOI: 10.1109/ISBI.2007.356867

Abstract

The fast and direct measurement of leukocyte behaviour in microvessels in situ is possible by labelling them with fluorescent compounds and subsequently imaging them by confocal laser scanning microscopy. Using a high-speed single line scanning technique a time dependent trace of leukocytes in microvessels can be recorded (x-t scan). These x-t traces, which are orientated orthogonal to the blood flow, are characterized by distinct, but often noise-affected elliptical streaks, which carry information on speed and frequency of leukocytes crossing the scan line. Here we describe the combination of a confocal laser scanning system and an image analysis software package that allows rapid and reproducible estimation of leukocyte velocities in microvessels.

Item Type:Conference contribution (Poster)
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Computer Science Research Institute
Biomedical Sciences Research Institute > Molecular Medicine
Biomedical Sciences Research Institute > Molecular Medicine > Neuroscience & Neurodegenerative Diseases
Biomedical Sciences Research Institute > Molecular Medicine > Vascular Medicine
ID Code:3759
Deposited By:Dr Kurt Saetzler
Deposited On:01 Feb 2010 09:50
Last Modified:24 Mar 2011 11:34

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