Bigot, S., Lucas, L., Morrow, PJ, Mitchell, Christopher and Saetzler, Kurt (2007) Estimating leukocyte velocities from high-speed 1D line scans oriented orthogonal to blood flow. In: Biomedical Imaging: Macro to Nano, 2007. 4rd IEEE International Symposium on, Washington. UNSPECIFIED. 4 pp. [Conference contribution]
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The fast and direct measurement of leukocyte behaviour in microvessels in situ is possible by labelling them with fluorescent compounds and subsequently imaging them by confocal laser scanning microscopy. Using a high-speed single line scanning technique a time dependent trace of leukocytes in microvessels can be recorded (x-t scan). These x-t traces, which are orientated orthogonal to the blood flow, are characterized by distinct, but often noise-affected elliptical streaks, which carry information on speed and frequency of leukocytes crossing the scan line. Here we describe the combination of a confocal laser scanning system and an image analysis software package that allows rapid and reproducible estimation of leukocyte velocities in microvessels.
|Item Type:||Conference contribution (Poster)|
|Faculties and Schools:||Faculty of Life and Health Sciences|
Faculty of Life and Health Sciences > School of Biomedical Sciences
|Research Institutes and Groups:||Biomedical Sciences Research Institute|
Computer Science Research Institute
Biomedical Sciences Research Institute > Molecular Medicine
Biomedical Sciences Research Institute > Molecular Medicine > Neuroscience & Neurodegenerative Diseases
Biomedical Sciences Research Institute > Molecular Medicine > Vascular Medicine
|Deposited By:||Dr Kurt Saetzler|
|Deposited On:||01 Feb 2010 09:50|
|Last Modified:||24 Mar 2011 11:34|
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