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HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF AMPICILLIN AND CLOXACILLIN AND ITS APPLICATION TO AN INTRAMAMMARY VETERINARY PREPARATION

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

BURNS, DT, O'CALLAGHAN, M, Smyth, Franklin and AYLING, CJ (1991) HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF AMPICILLIN AND CLOXACILLIN AND ITS APPLICATION TO AN INTRAMAMMARY VETERINARY PREPARATION. FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY, 340 (1). pp. 53-56. [Journal article]

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Abstract

Reverse phase high-performance liquid chromatography with ultra-violet detection has been used for the simultaneous identification and determination of ampicillin, cloxacillin and some of their degradation products following their extraction from an intramammary preparation used in veterinary medicine. Extraction of the intramammary base into petroleum ether (40-60-degrees-C) and partition of the penicillins into the mobile phase gave a mean recovery of 100.8% for ampicillin with coefficient of variation of 1.0 and 103.4% for cloxacillin with coefficient of variation of 1.2 (n = 6). Using optimised HPLC conditions ampicillin eluted in approximately 2 min and cloxacillin in approximately 8 min. The overall method was found to be stability indicating, since cloxacillin and ampicillin eluted independently of their degradation products.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Pharmaceutical Science and Practice
ID Code:3734
Deposited By:Prof W.Franklin Smyth
Deposited On:12 Jan 2010 09:34
Last Modified:04 May 2012 09:19

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