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Investigation of the electrochemical oxidation of clenbuterol at a porous carbon electrode, and its application to the determination of this beta-agonist in bovine hair by liquid chromatography with coulometric detection

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

McGrath, GJ, O'Kane, E, Smyth, Franklin and Tagliaro, F (1996) Investigation of the electrochemical oxidation of clenbuterol at a porous carbon electrode, and its application to the determination of this beta-agonist in bovine hair by liquid chromatography with coulometric detection. ANALYTICA CHIMICA ACTA, 322 (3). pp. 159-166. [Journal article]

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Abstract

At pH 5, clenbuterol is oxidized at potentials > 650 mV to form a radical cation, two of which combine in a head to head manner to form a dimer. A method for the determination of this notably abused beta(2)-adrenergic drug in bovine hair has been developed, utilizing this oxidative response. After alkaline degradation of the hair, the drug was extracted using a simple liquid-liquid extraction based on hexane, with a recovery of 92 +/- 3.6%. After evaporation to dryness, the residue is dissolved in mobile phase and the drug analysed by reversed phase liquid chromatography using a mobile phase consisting of methanol-0.1 M ammonium acetate (60:40, v/v) with 0.4% sodium dodecyl sulphate adjusted to pH 5 with orthophosphoric acid. Quantitation used a two cell coulometric detector with the first cell being used as a screening electrode and the analytical electrode set at +875 mV.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Pharmaceutical Science and Practice
ID Code:3723
Deposited By:Prof W.Franklin Smyth
Deposited On:12 Jan 2010 09:42
Last Modified:04 May 2012 09:19

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