Ulster University Logo

Ulster Institutional Repository

Competence for assembly of sister chromatid cores is progressively acquired during S phase in mammalian cells

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Gimenez-Abian, JF, Clarke, DJ, de la Torre, C, Gimenez-Martin, G, Mullinger , AM, Downes, Stephen and Johnson, RT (1999) Competence for assembly of sister chromatid cores is progressively acquired during S phase in mammalian cells. EUROPEAN JOURNAL OF CELL BIOLOGY, 78 (8). pp. 601-603. [Journal article]

Full text not available from this repository.

Abstract

Condensed sister chromatids possess a protein scaffold or axial core to which loops of chromatin are attached. The sister cores are believed to be dynamic frameworks that function in the organization and condensation of chromatids. Chromosome structural proteins are implicated in the establishment of sister chromatid cohesion and in the maintenance of epigenetic phenomena. Both processes of templating are tightly linked to DNA replication itself. It is a question whether the structural basis of sister chromatid cores is templated during S phase. As cells proceed through the cell cycle, chromatid cores undergo changes in their protein composition. Cytologically, cores are first visualized at the start of prometaphase, Still, core assembly can be induced in G(1) and G(2) when interphase cells are fused with mitotic cells. In this study, we asked if chromatid cores are similarly able to assemble in S-phase cells. We find that the ability to assemble cores is transiently lost during local replication, then regained in chromosome regions shortly after they have been replicated. We propose that core templating occurs coincident with DNA replication and that the competence for the assembly of the sister chromatid cores is acquired shortly after passage of replication forks.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Molecular Medicine
Biomedical Sciences Research Institute > Molecular Medicine > Nano Systems Biology
ID Code:3507
Deposited By:Professor Stephen Downes
Deposited On:15 Dec 2009 14:34
Last Modified:10 Jun 2010 12:11

Repository Staff Only: item control page