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Characterization of a mutant rat kangaroo cell line with alterations in the cell cycle and DNA repair

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Miyaji, EN, Johnson, RT, Downes, Stephen, Eveno, E, Mezzina, M, Sarasin, A and Menck, CFM (2000) Characterization of a mutant rat kangaroo cell line with alterations in the cell cycle and DNA repair. GENETICS AND MOLECULAR BIOLOGY, 23 (3). pp. 689-694. [Journal article]

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Abstract

Using a positive selection system for isolating DNA replication and repair related mutants, we isolated a clone from a rat kangaroo cell line (PtK2) that has increased sensitivity to UV light. Characterization of this clone indicated normal post-replication repair after UV irradiation, and normal removal rates of cyclobutane pyrimidine dimers and pyrimidine(6-4)pyrimidone photoproducts by excision repair. However, this cell line has decreased ability to make early incisions on damaged DNA, possibly indicating a defect in preferential repair of actively transcribed genes, and a slower cell proliferation rate, including a longer S-phase. This phenotype reinforces the present notion that control of key mechanisms in cell metabolism, such as cell cycle control, repair, transcription and cell death, can be linked.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Molecular Medicine
Biomedical Sciences Research Institute > Molecular Medicine > Nano Systems Biology
ID Code:3397
Deposited By:Professor Stephen Downes
Deposited On:15 Dec 2009 11:55
Last Modified:10 Jun 2010 12:02

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