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Elevated levels of oxidative DNA damage in lymphocytes from patients with Alzheimer's disease

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Morocz, M, Kalman, J, Juhasz, A, Sinko, I, McGlynn, AP, Downes, Stephen, Janka, Z and Rasko, I (2002) Elevated levels of oxidative DNA damage in lymphocytes from patients with Alzheimer's disease. NEUROBIOLOGY OF AGING, 23 (1). pp. 47-53. [Journal article]

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Abstract

Previous studies have provided evidence of the involvement of oxidative damage in the pathogenesis of Alzheimer's disease (AD). Although the role of oxidative stress in the aetiology of the disease is still not clear, the detection of an increased damage status in the cells of patients could have important therapeutic implications. The level of oxidative damage and repair capacity in peripheral lymphocytes of AD patients and of age-matched controls was determined by the Comet assay applied to freshly isolated blood samples with oxidative lesion-specific DNA repair endonucleases. This is less prone to errors arising from oxidative artifacts than chemical analytical methods; and is therefore a relatively reliable, as well as rapid method for assay of oxidative DNA damage in cells. Statistically significant elevations (P < 0.05) of oxidized purines were observed in nuclear DNA of peripheral lymphocytes from AD patients, compared. to age matched control subjects, both at basal level and after oxidative stress induced by H2O2. AD patients also showed a diminished repair of H2O2 -induced oxidized purines. (C) 2002 Elsevier Science Inc. All rights reserved.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Molecular Medicine
Biomedical Sciences Research Institute > Molecular Medicine > Nano Systems Biology
ID Code:3395
Deposited By:Professor Stephen Downes
Deposited On:15 Dec 2009 11:49
Last Modified:19 Oct 2010 14:38

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