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INDUCTION OF RAT HEPATIC MIXED-FUNCTION OXIDASES BY ACETONE AND OTHER PHYSIOLOGICAL KETONES - THEIR ROLE IN DIABETES-INDUCED CHANGES IN CYTOCHROME P450 PROTEINS

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Barnett, CR, Petrides, L, Wilson, J, Flatt, Peter and Ioannides, C (1992) INDUCTION OF RAT HEPATIC MIXED-FUNCTION OXIDASES BY ACETONE AND OTHER PHYSIOLOGICAL KETONES - THEIR ROLE IN DIABETES-INDUCED CHANGES IN CYTOCHROME P450 PROTEINS. XENOBIOTICA, 22 (12). pp. 1441-1450. [Journal article]

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Abstract

1. To evaluate the role of ketone bodies in diabetes-induced changes in hepatic cytochrome P450 composition, rats were treated with acetone, 3-hydroxybutyrate or 1,3-butanediol. 2. Treatment with acetone enhanced the rat hepatic 0-dealkylations of ethoxyresorufin and methoxyresorufin, and the hydroxylation of p-nitrophenol, but had no effect on lauric acid hydroxylation and ethylmorphine N-demethylation. Neither 3-hydroxybutyrate nor 1,3-butanediol modulated the metabolism of the above substrates. 3. Immunoblot analysis of hepatic microsomal proteins revealed that treatment with acetone increased the apoprotein levels of P4501A2, P4502B1/2 and P4502E1. 4. It is concluded that acetone is responsible, at least partly, for the diabetes-induced increase in hepatic microsomal P4501A2, P4502B1/2 and P4502E1 proteins but does not mediate the increases in the P4503A1 and P4504A1 proteins. On the basis of work from our own and other laboratories a mechanism for the diabetes-induced changes in hepatic cytochrome P450 proteins is proposed.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Diabetes
ID Code:3185
Deposited By:Professor Peter Flatt
Deposited On:08 Jan 2010 12:07
Last Modified:22 Jul 2011 14:21

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