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Hexose recognition by insulin-secreting BRIN-BD11 cells

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

McClenaghan, Neville, Gallagher, Alison, Barnett, CR and Flatt, Peter (1996) Hexose recognition by insulin-secreting BRIN-BD11 cells. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 223 (3). pp. 724-728. [Journal article]

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Abstract

Clonal BRIN-BD11 cells were produced by electrofusion of NEDH rat islet B-cells with immortal RINm5F cells. Western blotting analysis revealed that unlike RINm5F, novel BRIN-BD11 cells expressed high levels of the glucose transport protein GLUT-2, coupled with a rapid and sustained uptake for D-glucose, significantly greater than RINm5F after only 5 min (p<0.05). Whereas BRIN-BD11 cells expressed a high glucokinase:hexokinase ration with 1.4-2.0 fold and 1.4-1.7 fold stepwise stimulation of insulin secretion with 4.2-16.7 nM D-glucose and D-mannose respectively. RINm5F had a lower glucokinase;hexokinase ratio (p<0.001) and were notably unresponsive to D-glucose and D-mannose. Unlike RINm5F cells, BRIN-BD11 were unresponsive to other hexoses, with RINm5F only responding to D-galactose (p<0.05). BRIN-BD11 cells should be useful for studies of nutrient-induced insulin secretion. (C) 1996 Academic Press, Inc.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Diabetes
ID Code:3159
Deposited By:Professor Peter Flatt
Deposited On:08 Jan 2010 13:26
Last Modified:15 Jun 2011 11:10

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