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Functional examination of microencapsulated bioengineered insulin-secreting beta-cells

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Hamid, M, McCluskey, Janie, McClenaghan, Neville and Flatt, Peter (2001) Functional examination of microencapsulated bioengineered insulin-secreting beta-cells. CELL BIOLOGY INTERNATIONAL, 25 (6). pp. 553-556. [Journal article]

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Abstract

Clonal insulin-secreting BRIN-BD11 cells engineered by electrofusion were encapsulated inside natrium alginate beads and cultured in RPMI 1640 culture media. Acute insulin secretory responses to glucose and amino acids were compared between microencapsulated cells and non-encapsulated cells maintained in monolayer culture. Encapsulated cells exhibited a 1.5-fold, 2.9-fold and 4.2-fold increase (P>;0.001) in insulin release in response to 16.7 mmol/l glucose, 10 mmol/l L-arginine and 10 mmol/l L-alanine respectively. Insulin output by non-encapsulated cells was approximately 30% greater but the relative magnitudes of responses were similar. This is the first study to demonstrate the stability of cellular engineered insulin-secreting cells encapsulated in alginate beads, illustrating the utility of this approach for cellular engineering and potential transplantation in diabetes. (C) 2001 Academic Press.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Diabetes
ID Code:3080
Deposited By:Professor Peter Flatt
Deposited On:14 Jan 2010 09:35
Last Modified:15 Jun 2011 11:10

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