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Evaluation of glycated insulin in diabetic animals using immunocytochemistry and radioimmunoassay

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McKillop, Aine, Mooney, MH, Harriott, P, Flatt, Peter and O'Harte, Finbarr (2001) Evaluation of glycated insulin in diabetic animals using immunocytochemistry and radioimmunoassay. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 286 (3). pp. 524-528. [Journal article]

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Abstract

Glycated insulin was evaluated in plasma and biological tissues of diabetic animal models by immuno. cytochemistry (ICC) and a novel radioimmunoassay. Glycated insulin circulated at 0.10 +/-0.04 ng/ml and 2.20 +/-0.14 ng/ml in lean and diabetic obese (ob/ob) mice, corresponding to 12.5 and 9.8% total plasma insulin, respectively. The concentration of glycated insulin was elevated 22-fold in obese mice compared to controls (P<0.001). In the pancreas, glycated insulin was 48 +/-10 and 83 +/- 4 ng/g wt (P<0.05) in lean and obese mice, respectively, representing approximately 2% total insulin in the diabetic pancreas (4.60 plus/minus 0.17 mug/g wt). ICC revealed fluorescent positively stained cells in pancreatic islets from hydrocortisone (HC)treated diabetic rats. Fasting of HC-treated rats, resulted in 3-fold and 15-fold reductions in plasma glycated insulin (P<0.01) and insulin (P<0.001), respectively. Following a 30 min feeding period in these insulin resistant rats, plasma glucose, insulin, and glycated insulin increased (P<0.001) rapidly with 1.4-, 1.6-, and 2.9-fold elevations, respectively. Injection of HC-treated rats with insulin (50 U/kg) resulted in a rapid 33% decrease of plasma glucose (P<0.001) and a marked 4-fold increase in plasma insulin (P<0.01), whereas glycated insulin concentrations remained unchanged. Since glycation of insulin impairs biological activity, physiologically regulated secretion of glycated insulin into the circulation in diabetic animal models suggests a role in the pathogenesis of diabetes. (C) 2001 Academic Press.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
ID Code:3079
Deposited By:Professor Peter Flatt
Deposited On:14 Dec 2009 16:39
Last Modified:28 Feb 2011 11:19

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