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Time-correlation between membrane depolarization and intracellular calcium in insulin secreting BRIN-BD11 cells: studies using FLIPR

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Miguel, JC, Patterson, Steven, Abdel-Wahab, Yasser, Mathias, PCF and Flatt, Peter (2004) Time-correlation between membrane depolarization and intracellular calcium in insulin secreting BRIN-BD11 cells: studies using FLIPR. CELL CALCIUM, 36 (1). pp. 43-50. [Journal article]

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DOI: 10.1016/j.ceca.2003.11.007

Abstract

Cytoplasmic Ca2+ ([Ca2+](i)) and membrane potential changes were measured in clonal pancreatic beta cells using a fluorimetric imaging plate reader (FLIPR). KCl (30 mM) produced a fast membrane depolarization immediately followed by increase of [Ca2+](i) in BRIN-BD11 cells. L-Alanine (10 mM) but not L-arginine (10 mM) mimicked the KCl profile and also produced a fast membrane depolarization and elevation of [Ca2+];. Conversely, a rise in glucose from 5.6 mM to 11.1 or 16.7 mM induced rapid membrane depolarization, followed by a slower and delayed increase of [Ca2+](i). GLP-1 (20 nM) did not affect membrane potential or [Ca2+](i). In contrast, acetylcholine (ACh, 100 muM) induced fast membrane depolarization immediately followed by a modest [Ca2+]; increase. When extracellular Ca2+ was buffered with EGTA, ACh mobilized intracellular calcium stores and the [Ca2+]; increase was reduced by 2-aminoethoxydiphenyl borate but not by dantrolene, indicating the involvement of inositol triphosphate receptors (InsP(3)R). It is concluded that membrane depolarization of beta cells by glucose stimulation is not immediately followed by elevation of [Ca2+]i and other metabolic events are involved in glucose induced stimulus-secretion coupling. It is also suggested that ACh mobilizes intracellular Ca2+ through store operated InsP(3)R. (C) 2003 Elsevier Ltd. All rights reserved.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Diabetes
ID Code:3022
Deposited By:Professor Peter Flatt
Deposited On:14 Jan 2010 15:43
Last Modified:11 Jun 2010 11:21

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