Curragh, HJ, Mooibroek, H, Wessels, JGH, Marchant, R and Mullan, E (1993) PROTOPLAST FORMATION AND DNA-MEDIATED TRANSFORMATION OF FUSARIUM-CULMORUM TO HYGROMYCIN-B RESISTANCE. MYCOLOGICAL RESEARCH, 97 (Part 3). pp. 313-317. [Journal article]
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This work involved firstly optimizing the protoplast yields for F. culmorum 159026, then setting up a system for DNA-mediated transformation. Higher protoplast yields and more rapid regeneration were obtained when the organic stabilizers sucrose and sorbitol were used rather than NH4Cl. Successful transformation was achieved using the plasmids pAN7-1 and pHRC containing the E. coli hygromycin B resistance gene (hygromycin B phosphotransferase, hpt) fused to Aspergillus nidulans expression signals.
|Item Type:||Journal article|
|Faculties and Schools:||Faculty of Life and Health Sciences > School of Biomedical Sciences|
|Research Institutes and Groups:||Biomedical Sciences Research Institute|
|Deposited By:||Professor Roger Marchant|
|Deposited On:||15 Dec 2009 09:31|
|Last Modified:||27 Jun 2011 13:40|
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