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DIHYDROFOLATE-REDUCTASE SYNTHESIS IN CONTINUOUS-CULTURE USING A METHOTREXATE-RESISTANT ESCHERICHIA-COLI

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Singh - Nee Nigam, Poonam, Banat, Ibrahim, Kelly, BA and Marchant, R (1993) DIHYDROFOLATE-REDUCTASE SYNTHESIS IN CONTINUOUS-CULTURE USING A METHOTREXATE-RESISTANT ESCHERICHIA-COLI. ENZYME AND MICROBIAL TECHNOLOGY, 15 (8). pp. 652-656. [Journal article]

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Abstract

A methotrexate-resistant strain of Escherichia coli Type I produced exceptionally high levels of the enzyme dihydrofolate reductase (EC 1.5.1.3) in 6 h of batch fermentation. The culture had markedly improved performance under chemostat culture conditions in terms of enzyme yield and output. Temperature, pH, dilution rates, and nutrient composition were optimized under chemostat culture conditions. A maximum enzyme yield of 10,360 U l-1 and a specific activity of 22.84 U mg-1 were obtained under chemostat conditions at PH 7.0, temperature 37-degrees-C, and dilution rate 0.2 h-1, using media containing 1.0 and 0.6% (w/v) dextrose and yeast extract, respectively. The culture's performance and enzyme yields in chemostat and the feasibility of large-scale production are discussed.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Infection and Immunity/Microbiology
Biomedical Sciences Research Institute > Pharmaceutical Science and Practice
ID Code:2804
Deposited By:Professor Roger Marchant
Deposited On:15 Dec 2009 09:38
Last Modified:16 May 2012 14:26

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