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STUDIES ON THE GROWTH OF A THERMOTOLERANT YEAST-STRAIN, KLUYVEROMYCES-MARXIANUS IMB3, ON SUCROSE CONTAINING MEDIA

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Fleming, M, Barron, N, McHale, L, Marchant, Roger and McHale, Anthony (1993) STUDIES ON THE GROWTH OF A THERMOTOLERANT YEAST-STRAIN, KLUYVEROMYCES-MARXIANUS IMB3, ON SUCROSE CONTAINING MEDIA. BIOTECHNOLOGY LETTERS, 15 (12). pp. 1195-1198. [Journal article]

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Abstract

A thermotolerant alcohol-producing yeast strain, Kluyveromyces marxianus IMB3 was shown to grow on sucrose (10% [w/v]) containing media at 45-degrees-C. Under such conditions the organism reached stationary phase within 20 hours and yielded ethanol concentrations in the region of 33g/L. During growth on sucrose containing media the organism was found to produce a cell- associated activity capable of hydrolysing sucrose. This activity was shown to have a Km of 5.0mM when sucrose was used as the substrate. In addition the enzyme was shown to have a pH optimum of 5.0 and a temperature optimum of 50-55-degrees-C and under those conditions the enzyme was shown to be relatively thermostable.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Faculty of Life and Health Sciences > School of Pharmacy and Pharmaceutical Science
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Pharmaceutical Science and Practice
ID Code:2803
Deposited By:Professor Roger Marchant
Deposited On:15 Dec 2009 10:24
Last Modified:04 Dec 2012 12:02

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