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PARTIAL CHARACTERIZATION OF BETA-GLUCOSIDASE ACTIVITY PRODUCED BY KLUYVEROMYCES-MARXIANUS IMB3 DURING GROWTH ON CELLOBIOSE-CONTAINING MEDIA AT 45-DEGREES-C

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BARRON, N, Marchant, R, MCHALE, L and McHale, Anthony (1995) PARTIAL CHARACTERIZATION OF BETA-GLUCOSIDASE ACTIVITY PRODUCED BY KLUYVEROMYCES-MARXIANUS IMB3 DURING GROWTH ON CELLOBIOSE-CONTAINING MEDIA AT 45-DEGREES-C. BIOTECHNOLOGY LETTERS, 17 (10). pp. 1047-1050. [Journal article]

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Abstract

We report- the partial characterization of a beta-glucosidase produced during growth of the thermotolerant yeast, K. marxianus IMB3 on lactose-containing media at 45 degrees C. The enzyme had Km values of 1.1 mM and 14.8 mM, for the substrates p-nitrophenyl-beta-D-glucoside and cellobiose, respectively. The enzyme had a pH optimum of 5.5 and was optimally active at 50 degrees C. It was stable up to 125 hours at 25 degrees C and 35 degrees, with half-lives of 45 hours and 2 hours at 45 degrees C and 50 degrees C, respectively. inactivated by Hg2+. Ethanol concentrations [1-10% (v/v)] had little effect an activity. Glucose (20 mM) caused inhibition when p-nitrophenyl-beta-D-glucoside was used as substrate, whereas lactose at similar concentrations had no effect.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Faculty of Life and Health Sciences > School of Pharmacy and Pharmaceutical Science
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Pharmaceutical Science and Practice
ID Code:2789
Deposited By:Professor Roger Marchant
Deposited On:15 Dec 2009 10:24
Last Modified:04 Dec 2012 11:53

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