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Physiological and biochemical characterization of intergeneric hybrids of thermotolerant and non-thermotolerant yeasts

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Dhamija, SS, Gera, R, Singh, DK, Singh, D and Marchant, R (1997) Physiological and biochemical characterization of intergeneric hybrids of thermotolerant and non-thermotolerant yeasts. JOURNAL OF BASIC MICROBIOLOGY, 37 (5). pp. 307-312. [Journal article]

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Abstract

Kluyveromyces-like intergeneric hybrids of thermotolerant Kluyveromyces marxianus and non-thermo-tolerant Saccharomyces cerevisiae, produced in a previous study by protoplasmic fusion, have been characterized. On molasses, these strains produced ethanol in excess of 6% (v/v) both at 30 and 45 degrees C as against 3% and 4.2% (v/v) by the former parent at 30 and 45 degrees C, respectively. In hybrids, the increase in ethanol production appeared to be a sequel to increased activities of alcohol dehydrogenase and pyruvate kinase, derived probably from S. cerevisiae parent. Hybrid ADH-isozyme pattern on polyacrylamide gel corroborated the presence of S. cerevisiae ADH in the tested hybrids. Regression analyses indicated a positive correlation between ethanol production and ADH or PK or both (r similar to 0.76-0.84).

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
ID Code:2761
Deposited By:Professor Roger Marchant
Deposited On:15 Dec 2009 16:20
Last Modified:15 Oct 2012 09:17

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