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Improving the quality of industrially important enzymes by directed evolution

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Chirumamilla, RR, Muralidhar, R, Marchant, R and Singh - Nee Nigam, Poonam (2001) Improving the quality of industrially important enzymes by directed evolution. MOLECULAR AND CELLULAR BIOCHEMISTRY, 224 (1-2). pp. 159-168. [Journal article]

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Abstract

Directed evolution is a new process for developing industrially viable biocatalysts. This technique does not require a comprehensive knowledge of the relationships between sequence structure and function of proteins as required by protein engineering. It mimics the process of Darwinian evolution in a test tube combining random mutagenesis and recombination with screening or selection for enzyme variants that have the desired properties. Directed evolution helps in enhancing the enzyme performance both in natural and synthetic environments. This article reviews the process of directed evolution and its application to improve substrate specificity, activity, enantioselectivity and thermal stability.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Infection and Immunity/Microbiology
ID Code:2738
Deposited By:Professor Roger Marchant
Deposited On:15 Dec 2009 16:31
Last Modified:04 Aug 2011 09:47

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