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Fabrication of impedimetric sensors for labe-free Point-of-Care immunoassay cardiac marker systems with microfluidic blood flow delivery

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Tweedie, M, Subramanian, R, Lemoine, P, Craig, I, McAdams, ET and McLaughlin, JAD (2006) Fabrication of impedimetric sensors for labe-free Point-of-Care immunoassay cardiac marker systems with microfluidic blood flow delivery. In: EMBS 2006, New York, USA. UNSPECIFIED. 5 pp. [Conference contribution]

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URL: http://www.ieeexplore.ieee.org/search/srchabstract.jsp?arnumber=4462829&isnumber=4461641&punumber=4028925&k2dockey=4462829@ieeecnfs&query=%28mclaughlin+%3Cin%3E+au%29+%3Cand%3E+%284461641+%3Cin%3E+isnumber%29&pos=0&access=no

DOI: 10.1109/IEMBS.2006.260742

Abstract

Miniaturised point-of-care cardiac marker sensors are being developed, based on impedimetric sensing of cardiac enzyme capture by an antibody layer immobilised on a planar gold electrode sensor. Gold/Ti-on-glass substrates have been used, in a 2 electrode configuration, with antibodies immobilised on the working electrode. Microfluidic structures have been fabricated by a CO2 laser, in 25 mum thick pressure sensitive adhesive (PSA), on a PMMA lid, and the structure bonded on top of the planar sensor. Microfluidic blood/serum delivery has been investigated using a visualisation dye. Some flow problems are observed if the sensor is exposed to air for several days, suggesting that flow channel nanopillars and hermetic encapsulation may be required to guarantee flow properties in commercially produced modules. Work is ongoing to characterise the impedimetric signal changes for myoglobin capture by antimyoglobin, using these sensors. Fifty micron thick PSA, incorporating a robust spacer layer, will be used to give better definition of channel walls

Item Type:Conference contribution (Paper)
Keywords:Impedimetric antibody cardiac microfluidic planar point-of-care sensor
Faculties and Schools:Faculty of Computing & Engineering
Faculty of Computing & Engineering > School of Engineering
Research Institutes and Groups:Engineering Research Institute
Engineering Research Institute > Nanotechnology & Integrated BioEngineering Centre (NIBEC)
ID Code:2449
Deposited By:Mrs Ann Blair
Deposited On:04 Dec 2009 13:37
Last Modified:15 Jun 2011 10:52

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