Ulster University Logo

Ulster Institutional Repository

Endocytic Structures and Synaptic Vesicle Recycling at a Central Synapse in Awake Rats.

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Körber, Christoph, Horstmann, Heinz, Saetzler, Kurt and Kuner, Thomas (2012) Endocytic Structures and Synaptic Vesicle Recycling at a Central Synapse in Awake Rats. Traffic, 13 . 1601–1611. [Journal article]

[img]PDF - Published Version
Indefinitely restricted to Repository staff only.

29Mb

URL: http://dx.doi.org/10.1111/tra.12007

DOI: 10.1111/tra.12007

Abstract

The synaptic vesicle (SV) cycle has been studied extensively in cultured cells and slice preparations, but not much is known about the roles and relative contributions of endocytic pathways and mechanisms of SV recycling in vivo, under physiological patterns of activity. We employed horseradish peroxidase (HRP) as an in vivo marker of endocytosis at the calyx of Held synapse in the awake rat. Ex vivo serial section scanning electron microscopy and 3D reconstructions revealed two categories of labelled structures: HRP-filled SVs and large cisternal endosomes. Inhibition of adaptor protein complexes 1 and 3 (AP-1, AP-3) by in vivo application of Brefeldin A (BFA) disrupted endosomal SV budding while SV recycling via clathrin-mediated endocytosis (CME) remained unaffected. In conclusion, our study establishes cisternal endosomes as an intermediate of the SV cycle and reveals CME and endosomal budding as the predominant mechanisms of SV recycling in a tonically active central synapse in vivo.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Molecular Medicine
ID Code:24317
Deposited By:Dr Kurt Saetzler
Deposited On:11 Dec 2012 10:24
Last Modified:11 Dec 2012 10:24

Repository Staff Only: item control page