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PROTEOMIC IDENTIFICATION OF A BIOMARKER CLUSTER ASSOCIATED WITH RECURRENT JOINT INFLAMMATION IN JUVENILE IDIOPATHIC ARTHRITIS

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Gibson, David (2005) PROTEOMIC IDENTIFICATION OF A BIOMARKER CLUSTER ASSOCIATED WITH RECURRENT JOINT INFLAMMATION IN JUVENILE IDIOPATHIC ARTHRITIS. In: Irish Society for Rheumatology and Irish Rheumatology Health Professionals Society, Annual General Meeting , Trinity Centre – St. James’s Hospital Dublin 8. Springer. Vol 174 (4) 1 pp. [Conference contribution]

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Abstract

The synovial proteome was investigated to isolate joint-specific biomarkers that are significantly expressed in Juvenile idiopathic arthritis (JIA) patients displaying recurrent inflammation. These proteins could act as novel therapeutic targets. JIA comprises a poorly understood group of chronic autoimmune diseases with variable clinical presentations and outcomes.Serial synovial fluid and matched plasma samples (n=6 patients) were subjected to protein separation by 2-dimension electrophoreisis (2DE). Focused gels were fixed, stained with colloidal Coomassie, scanned and protein spot intensities quantified by Phoretics software analysis. Pooled subgroup profiles (n=12 patients) were used to highlight joint specific protein spots with expression patterns that correlated with recurrence of joint inflammation. Protein spots were picked, digested by trypsin and extracted. Peptides (n=3) were identified with a mass spectrometer.2DE reveals ~680 spots per gel within the pH 4-7 range for synovial fluid and plasma. On comparison of plasma and synovial ‘master’ gels, a subpopulation of spots uniquely expressed in synovial fluid, were identified. The expression levels of 12 synovial protein spots correlated positively with clinical and laboratory indices of inflammation. Furthermore they were over represented in those patients with recurrent joint effusions (p<0.01). Proteolytic fragments of collagen X, fibrin β-chain and T-cell receptor α-region were differentially expressed amongst this subpopulation in JIA.Protein spots unique to the synovial joint and furthermore differentially expressed in patients with recurrent joint inflammation have been isolated and identified by 2DE-MS. These proteins may play a significant role determining the pathophysiological state within the chronically inflamed joint and influence JIA disease progression.

Item Type:Conference contribution (Speech)
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Stratified Medicine
ID Code:24206
Deposited By:Dr David Gibson
Deposited On:30 Nov 2012 13:54
Last Modified:15 Oct 2013 16:09

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