Gibson, David, Newell, Keri, Evans, Alexandra, Finnegan, Sorcha, Manning, Gwen, Scaife, Caitiona, McAllister, Catherine, Pennington, Stephen, Duncan, Mark, Moore, Terry and Rooney, Madeleine (2012) Vitamin D binding protein isoforms as candidate predictors of disease extension in childhood arthritis. Journal of Proteomics, 75 . pp. 5479-5492. [Journal article]
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Introduction. Juvenile idiopathic arthritis (JIA) comprises a poorly understood group ofchronic autoimmune diseases with variable clinical outcomes. We investigated whetherthe synovial fluid (SF) proteome could distinguish a subset of patients in whom diseaseextends to affect a large number of joints.Methods. SF samples from 57 patients were obtained around time of initial diagnosis of JIA,labeled with Cy dyes and separated by two-dimensional electrophoresis. Multivariateanalyses were used to isolate a panel of proteins which distinguish patient subgroups.Proteins were identified using MALDI-TOF mass spectrometry with expression verified byimmunochemical methods. Protein glycosylation status was confirmed by hydrophilicinteraction liquid chromatography.Results. A truncated isoform of vitamin D binding protein (VDBP) is present at significantlyreduced levels in the SF of oligoarticular patients at risk of disease extension, relative toother subgroups (p<0.05). Furthermore, sialylated forms of immunopurified synovial VDBPwere significantly reduced in extended oligoarticular patients (p<0.005).Conclusion. Reduced conversion of VDBP to a macrophage activation factor may be used tostratify patients to determine risk of disease extension in JIA patients.
|Item Type:||Journal article|
|Faculties and Schools:||Faculty of Life and Health Sciences|
Faculty of Life and Health Sciences > School of Biomedical Sciences
|Research Institutes and Groups:||Biomedical Sciences Research Institute|
Biomedical Sciences Research Institute > Stratified Medicine
|Deposited By:||Dr David Gibson|
|Deposited On:||08 Oct 2012 09:40|
|Last Modified:||12 Feb 2014 12:06|
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