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Vitamin D binding protein isoforms as candidate predictors of disease extension in childhood arthritis

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Gibson, David, Newell, Keri, Evans, Alexandra, Finnegan, Sorcha, Manning, Gwen, Scaife, Caitiona, McAllister, Catherine, Pennington, Stephen, Duncan, Mark, Moore, Terry and Rooney, Madeleine (2012) Vitamin D binding protein isoforms as candidate predictors of disease extension in childhood arthritis. Journal of Proteomics, 75 . pp. 5479-5492. [Journal article]

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DOI: 10.1016/j.jprot.2012.06.024


Introduction. Juvenile idiopathic arthritis (JIA) comprises a poorly understood group ofchronic autoimmune diseases with variable clinical outcomes. We investigated whetherthe synovial fluid (SF) proteome could distinguish a subset of patients in whom diseaseextends to affect a large number of joints.Methods. SF samples from 57 patients were obtained around time of initial diagnosis of JIA,labeled with Cy dyes and separated by two-dimensional electrophoresis. Multivariateanalyses were used to isolate a panel of proteins which distinguish patient subgroups.Proteins were identified using MALDI-TOF mass spectrometry with expression verified byimmunochemical methods. Protein glycosylation status was confirmed by hydrophilicinteraction liquid chromatography.Results. A truncated isoform of vitamin D binding protein (VDBP) is present at significantlyreduced levels in the SF of oligoarticular patients at risk of disease extension, relative toother subgroups (p<0.05). Furthermore, sialylated forms of immunopurified synovial VDBPwere significantly reduced in extended oligoarticular patients (p<0.005).Conclusion. Reduced conversion of VDBP to a macrophage activation factor may be used tostratify patients to determine risk of disease extension in JIA patients.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Stratified Medicine
ID Code:23513
Deposited By:Dr David Gibson
Deposited On:08 Oct 2012 09:40
Last Modified:07 Apr 2014 09:53

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