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Lipopeptide Biosurfactants from Paenibacillus polymyxa Inhibit Single and Mixed Species Biofilms

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Quinn, Gerry A., Maloy, Aaron P., McClean, Stephen, Carney, Brian and Slater, John W. (2012) Lipopeptide Biosurfactants from Paenibacillus polymyxa Inhibit Single and Mixed Species Biofilms. Biofouling, 28 (10). pp. 1151-1166. [Journal article]

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URL: http://www.tandfonline.com/doi/abs/10.1080/08927014.2012.738292

DOI: 10.1080/08927014.2012.738292

Abstract

Although biofilms are recognised as important in microbial colonisation, solutions to theirinhibition are predominantly based on planktonic assays. These solutions have limited efficacy against biofilms. Here, a series of biofilm-orientated tests were used to identify antibiofilm compounds from marine micro-flora. This led to the isolation of a complex of antibiofilm compounds from a Paenibacillus polymyxa extract (PPE). A combination of rpHPLC and mass spectrometry identified the principle components of PPE as fusaricidin B (LI-FO4b) and polymyxin D1 with minor contributions from surfactins. This complex (PPE) reduced the biofilm biomass of Bacillus subtilis, Micrococcus luteus, Pseudomonas aeruginosa, Staphylococcus aureus and Streptococcus bovis. In contrast, ampicillin was only effective against S. aureus. PPE also inhibited a self-assembling marine biofilm (SAMB) in co-incubation assays by 99.3% ±1.9 and disrupted established SAMB by 72.4% ± 4.4, while ampicillin showed no significant reduction. The effectiveness of this complex of lipopeptides against single and multispecies biofilms suggests a future role in biofilm prevention strategies.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Pharmaceutical Science and Practice
ID Code:23481
Deposited By:Dr Stephen McClean
Deposited On:01 Oct 2012 11:32
Last Modified:12 Nov 2012 12:30

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