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A novel vaginal ring device for the sustained delivery of recombinant C-clade HIV-1 CN54gp140

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Morrow, RJ, Donnelly, L, Curran, Rhonda M, Malcolm, RK, Woolfson, AD, Andrews, GP and Shattock, RJ (2009) A novel vaginal ring device for the sustained delivery of recombinant C-clade HIV-1 CN54gp140. Retrovirology, 6 (suppl 3) . p. 157. [Journal article]

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URL: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2767645/pdf/1742-4690-6-S3-P157.pdf

DOI: 10.1186/1742-4690-6-S3-P157

Abstract

BackgroundCervicovaginal (CV) tissue is the primary site for HIV transmission and a major reservoir for viral replication. Therefore, a successful vaccine strategy should induce potent immune responses at this mucosal surface. One potential method for achieving a localised immuneresponse is to administer the antigen directly to the CV tissue (Marx et al., 1993, Wassen et al., 1996). The aim of the study was to evaluate the in vitro release characteristics oflyophilised hydroxypropylmethylcellulose (HPMC) gel inserts containing HIV envelope protein CN54gp140 either as stand-alone formulations or located within a novel rod-insert vaginal ring device (RIVR).MethodsVaginal gel formulations comprising either low or high molecular weight (MW) HPMC and CN54gp140 (0.25% w/w) were prepared. The gels were injected into silicone elastomer tubing, cut to 40 mm lengths, and then lyophilised to produce solid rod inserts. In vitro release testing was performed on rod inserts alone and rod insert vaginal rings (RIVRs) containing two CN54gp140-loaded rod inserts. Release of gp140 was quantified by ELISA.ResultsRelease of CN54gp140 from rods prepared from low MW HPMC was similar for both inserts alone and rings containing inserts (99% cf. 90% over two hr). Release from the high MW inserts was significantly slower and more sustained than the low MW formulations. Also, CN54gp140 release from high MW rod inserts rings (68% of theoretical over 48 hr) was significantly more sustained than high MW inserts alone (86% over 24 hr).ConclusionThe study demonstrates that CN54gp140 (i) maintains its antigenicity during the preparation of HPMC lyophilised rod inserts, and (ii) may be administered in a sustainedmanner when the inserts are placed into an RIVR device. This stable, sustained-release formulation strategy has the potential to induce stronger immune responses andimmune memory upon vaginal administration (Zhao and Leong, 1996; Lofthouse, 2002).

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Nursing
Research Institutes and Groups:Institute of Nursing and Health Research
Institute of Nursing and Health Research > Maternal, Fetal and Infant Research
ID Code:23201
Deposited By:Dr Rhonda Curran
Deposited On:31 Aug 2012 11:58
Last Modified:31 Aug 2012 11:58

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