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Production of serotype C specific and serotype C/D generic monoclonalantibodies using recombinant HC and HN fragments from Clostridiumbotulinum neurotoxin types C1 and D

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Curran, Rhonda M, Fringuelli, E, Graham, David and Elliott, Chris T (2009) Production of serotype C specific and serotype C/D generic monoclonalantibodies using recombinant HC and HN fragments from Clostridiumbotulinum neurotoxin types C1 and D. Veterinary Immunology and Immunopathology, 130 . pp. 1-10. [Journal article]

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URL: http://ac.els-cdn.com/S0165242708007460/1-s2.0-S0165242708007460-main.pdf?_tid=7004fe04-f037-11e1-b459-00000aab0f27&acdnat=1346065849_7143ac54c137d66c7ab11174197f3ee5

DOI: 10.1016/j.vetimm.2008.11.018

Abstract

Sequence variability of Clostridium botulinum serotypes C and D is particularly complex.Some serotype C and D strains have unique gene structures that encode mosaic isoforms ofbotulinum neurotoxin (BoNT) containing components of both BoNT type C1 (BoNT/C1) andBoNT type D (BoNT/D). Such sequence variability and the potential for cross neutralisationmust be taken into consideration when developing serotype C and D detection andidentification assays. Three fusion proteins containing either a fragment from thecarboxyl-terminal domain of the heavy chain (HC) of BoNT/C1 (strain 573), a fragment fromthe HC of BoNT/D (strain BVD/-3) or a fragment from the amino-terminal domain of theheavy chain (HN) of BoNT/C1 (strain 573) were expressed in Escherichia coli, andadministered as immunogens to mice. Monoclonal antibodies (mAbs) against therecombinant BoNT fragments were prepared by three fusions. MAbs recognising nativeBoNT/C1 and BoNT/D were detected by enzyme-linked immunosorbent assay (ELISA). Ninemonoclonal antibodies (mAbs) were produced, six of which recognised a BoNT fragmentthat is highly conserved across all serotype C and D producing strains. We conclude thatthese mAbs and this approach to mAb production may facilitate the development ofimmunological diagnostic techniques that are not constrained by the existence of mosaicisoforms for the detection and identification of serotypes C and D.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Nursing
Research Institutes and Groups:Institute of Nursing and Health Research
Institute of Nursing and Health Research > Maternal, Fetal and Infant Research
ID Code:23194
Deposited By:Dr Rhonda Curran
Deposited On:04 Sep 2012 14:41
Last Modified:04 Sep 2012 14:41

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