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Development of liposome gel based formulations for intravaginal deliveryof the recombinant HIV-1 envelope protein CN54gp140

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Gupta, Prem N, Pattani, Aditya, Curran, Rhonda M, Kett, Vicky L, Andrews, Gavin P, Morrow, Ryan J, Woolfson, A David and Malcolm, R Karl (2012) Development of liposome gel based formulations for intravaginal deliveryof the recombinant HIV-1 envelope protein CN54gp140. European Journal of Pharmaceutical Sciences, 46 (5). pp. 315-322. [Journal article]

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URL: http://ac.els-cdn.com/S0928098712000619/1-s2.0-S0928098712000619-main.pdf?_tid=f1c7325556b3e7cfd3d49251de905a67&acdnat=1345814123_1b0fbe018d47e14c410112365abf3dec

DOI: 10.1016/j.ejps.2012.02.003

Abstract

Mucosally-administered vaccine strategies are widely investigated as a promising means of preventing HIV infection. This study describes the development of liposomal gel formulations, and novel lyophilised variants, comprising HIV-1 envelope glycoprotein, CN54gp140, encapsulated within neutral, positively charged or negatively charged liposomes. The CN54gp140 liposomes were evaluated for mean vesicle diameter, polydispersity, morphology, zeta potential and antigen encapsulation efficiency before being incorporated into hydroxyethyl cellulose (HEC) aqueous gel and subsequently lyophilised to produce a rod-shaped solid dosage form for practical vaginal application. The lyophilised liposome–HEC rods were evaluated for moisture content and redispersibility in simulated vaginal fluid. Since these rods are designed to revert to gel form following intravaginal application, mucoadhesive, mechanical (compressibility and hardness) and rheological properties of the reformed gels were evaluated. The liposomes exhibited good encapsulation efficiency and the gels demonstrated suitable mucoadhesive strength. The freezedried liposome–HEC formulations represent a novel formulation strategy that could offer potential as stable and practical dosage form.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Nursing
Research Institutes and Groups:Institute of Nursing and Health Research
Institute of Nursing and Health Research > Maternal, Fetal and Infant Research
ID Code:23179
Deposited By:Dr Rhonda Curran
Deposited On:04 Sep 2012 14:43
Last Modified:04 Sep 2012 14:43

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