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Circular Dichroism of Quadruplex Structures

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Randazzo, Antonio, Spada, Gian Piero and Webba da Silva, Mateus (2012) Circular Dichroism of Quadruplex Structures. In: Topics in Current Chemistry. Springer Verlag, Berlin Heidelberg, Berlin / Heidelberg, pp. 1-20. ISBN 0340-1022 [Book section]

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URL: http://www.springerlink.com/content/71430406151w0876/

DOI: 10.1007/128_2012_331

Abstract

Circular dichroism (CD) is a widespread technique for studying the polymorphism of G-quadruplexes. In this chapter the CD spectral features characteristic of different folding topologies of G4-DNA are analyzed in terms of the sequence of the syn or anti glycosidic bond angle (GBA) within a quadruplex stem. Depending on the GBA sequence, the chiral disposition of two stacked guanines, adjacent along a strand, is different and this leads to a predictable contribution to the overall CD spectrum. The CD spectra of a series of G-quadruplexes, chosen as prototypal of the most common strand folding, are illustrated. The validity and the prediction power of the approach is corroborated by the analysis of CD spectra of structurally modified G4-DNA either with chemically modified guanines or polarity inversion site (5-5 or 3-3) along the strands or additional nucleobases contributing to the stacking.

Item Type:Book section
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Pharmacy and Pharmaceutical Science
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Molecular Medicine
Biomedical Sciences Research Institute > Molecular Medicine > Transcriptional Regulation & Epigenetics
ID Code:22891
Deposited By:Dr Mateus Webba da Silva
Deposited On:24 Sep 2012 11:17
Last Modified:04 Dec 2012 14:18

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