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Verification of cell viability at progressively higher scanning forces using a hybrid atomic force and fluorescence microscope.

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Barnes, Clifford, O'Hagan, Barry, Howard, Vyvyan and McKerr, G (2007) Verification of cell viability at progressively higher scanning forces using a hybrid atomic force and fluorescence microscope. Journal of microscopy, 228 (Pt 2). pp. 185-9. [Journal article]

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Abstract

The prudent use of the atomic force microscope as a supra-vital live cell imaging tool requires that cell viability must be determined before and after scanning. Complementary optical techniques in conjunction with the fluorescent dyes rhodamine-123 and ethidium homodimer have been used within this study to determine cell viability after increasing loads are applied in contact mode. Guideline force ranges for five commonly cultured cell lines, human squamous carcinoma (A431), fibroblast, HeLa, Potorous tridactylis (PtK2) and rat intestinal epithelial (RIE) cells are given.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Molecular Medicine
ID Code:22106
Deposited By:Dr Barry O'Hagan
Deposited On:15 May 2012 11:49
Last Modified:15 May 2012 11:49

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