Ulster University Logo

Ulster Institutional Repository

Investigation of antibacterial phytochemicals in the bark and leaves of Ficus coronata by high-performance liquid chromatography-electrospray ionization-ion trap mass spectrometry (HPLC-ESI-MSn) and ESI-MSn

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Smyth, Thomas J. P., Ramachandran, Venkataraman, Brooks, Peter and Smyth, Franklin (2012) Investigation of antibacterial phytochemicals in the bark and leaves of Ficus coronata by high-performance liquid chromatography-electrospray ionization-ion trap mass spectrometry (HPLC-ESI-MSn) and ESI-MSn. ELECTROPHORESIS, 33 (4, SI). pp. 713-718. [Journal article]

Full text not available from this repository.

DOI: 10.1002/elps.201100302

Abstract

The ethyl acetate extracts of the bark and leaves of Ficus coronata were separated by column chromatography and the resulting fractions tested for their bioactivity toward methicillin-resistant-Staphylococcus aureus (MRSA) and M. luteus. The bioactive column chromatography fractions were further separated by preparative thin layer chromatography (TLC) and the resulting bands investigated by high-performance liquid chromatographyelectrospray ionizationion trap mass spectrometry (HPLC-ESI-MSn) and ESI-MSn. The resulting retention times, molecular masses, their fragmentation patterns, and the chemnet database () were then used in the dereplication process by structural elucidation of some of the compounds when compared with known structures of natural origin. Some molecular masses and the corresponding fragmentations were found that did not correlate with any known compounds thus revealing potentially novel natural products that could be investigated on a larger scale and could ultimately find application as new drugs against MRSA and other multidrug-resistant microorganisms. Structures are also proposed for known compounds that have not been previously reported for F. coronata.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Pharmaceutical Science and Practice
ID Code:22066
Deposited By:Prof W.Franklin Smyth
Deposited On:08 May 2012 11:56
Last Modified:08 May 2012 11:56

Repository Staff Only: item control page