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Graphene oxide integrated sensor for electrochemical monitoring ofmitomycin C–DNA interaction

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Erdem, A, Muti, M, Papakonstantinou, P, Canavar, E, Karadeniz, H, Congur, G and Sharma, S (2012) Graphene oxide integrated sensor for electrochemical monitoring ofmitomycin C–DNA interaction. ANALYST, 137 . pp. 2129-2135. [Journal article]

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URL: http://pubs.rsc.org/en/content/articlelanding/2012/an/c2an16011k/unauth

DOI: 10.1039/C2AN16011K

Abstract

We present a graphene oxide (GO) integrated disposable electrochemical sensor for the enhanced detection of nucleic acids and the sensitive monitoring of the surface-confined interactions between the anticancer drug mitomycin C (MC) and DNA. Interfacial interactions between immobilized calf thymus double-stranded (dsDNA) and anticancer drug MC were investigated using differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS) techniques. Based on three repetitive voltammetric measurements of 120 μg mL(-1) DNA immobilized on GO-modified electrodes, the RSD % (n = 3) was calculated as 10.47% and the detection limit (DL) for dsDNA was found to be 9.06 μg mL(-1). EIS studies revealed that the binding of the drug MC to dsDNA leads to a gradual decrease of its negative charge. As a consequence of this interaction, the negative redox species were allowed to approach the electrode, and thus increase the charge transfer kinetics. On the other hand, DPV studies exploited the decrease of the guanine signal due to drug binding as the basis for specifically probing the biointeraction process between MC and dsDNA

Item Type:Journal article
Faculties and Schools:Faculty of Computing & Engineering
Faculty of Computing & Engineering > School of Engineering
Research Institutes and Groups:Engineering Research Institute
Engineering Research Institute > Nanotechnology & Integrated BioEngineering Centre (NIBEC)
ID Code:21807
Deposited By:Professor Pagona Papakonstantinou
Deposited On:16 Apr 2012 10:47
Last Modified:16 Apr 2012 10:47

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