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A novel type of regulatory element is required for promoter-specific activity of the PDGF-B intronic enhancer region

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Miller, SJ, Ulleras, E, Moncrieff, CL, Walsh, Colum, Adam, GIR and Franklin, GC (1998) A novel type of regulatory element is required for promoter-specific activity of the PDGF-B intronic enhancer region. GROWTH FACTORS, 16 (2). pp. 137-151. [Journal article]

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Abstract

We have previously described a non-classical, promoter-specific enhancer for the human Platelet-Derived Growth Factor B (PDGF-B) gene. In JEG-3 choriocarcinoma cells the activity of the enhancer depends upon co-operation with a sequence (the Enhancer-Dependent cis Go-activator ``EDC'' element) within the promoter. The PDGF-B enhancer fails to activate heterologous promoters, indicating that promoter-specificity depends on an element within the enhancer that can recognise a target sequence within the promoter. Here we identify a sequence within the enhancer of the PDGF-B gene which directs activation of the PDGF-B promoter by distal cis-acting elements, This specifies the wild-type PDGF-B promoter as the target for the enhancer and has been designated the EDC specificity element (EDCse), The cell-type specific nature of this interaction is extended by the observation that the EDCse is also dispensable for enhancer activity in breast-cancer cells (ZR-75), Concomitant to this observation, JEG-3 and ZR-75 cells differ in the binding of nuclear factors to the EDCse, We discuss the relevance of the EDC/EDCse system in regulation of gene expression.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Molecular Medicine
Biomedical Sciences Research Institute > Molecular Medicine > Transcriptional Regulation & Epigenetics
ID Code:21732
Deposited By:Professor Colum Walsh
Deposited On:02 Apr 2012 11:10
Last Modified:11 Dec 2012 14:46

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