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Timing of establishment of paternal methylation imprints in the mouse

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

Li, JY, Lees Murdock, Diane, Xu, GL and Walsh, Colum (2004) Timing of establishment of paternal methylation imprints in the mouse. GENOMICS, 84 (6). pp. 952-960. [Journal article]

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DOI: 10.1016/j.ygeno.2004.08.012

Abstract

Imprinted genes are characterized by predominant expression from one parental allele and differential DNA methylation. Few imprinted genes have been found to acquire a methylation mark in the male germ line, however, and only one of these, H19, has been studied in detail. We examined methylation of the Rasgif1 and Gtl2 differentially methylated regions (DMR) to determine whether methylation is erased in male germ cells at c12.5 and when the paternal allele acquires methylation. We also compared their methylation dynamics with those of H19 and the maternally methylated gene Snrpn. Our results show that methylation is erased on Rasgrf1, H19, and Snrpn at e12.5, but that Gt12 retains substantial methylation at this stage. Erasure of methylation marks on Gt12 appears to occur later in female germ cells to give the unmethylated profile seen in mature MII oocytes. In the male germ line, de novo methylation of Rasgrf1, Gtl2, and H19 occurs in parallel between e12.5 and e17.5, but the DMR are not completely methylated until the mature sperm stage, suggesting a methylation dynamic different from that of IAP, L1, and minor satellite sequences, which have been shown to become fully methylated by e17.5 in male germ cells. This study also indicates important differences between different imprinted DMR in timing and extent of methylation in the germ cells. (C) 2004 Elsevier Inc. All rights reserved.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Molecular Medicine
Biomedical Sciences Research Institute > Molecular Medicine > Transcriptional Regulation & Epigenetics
ID Code:21713
Deposited By:Professor Colum Walsh
Deposited On:02 Apr 2012 10:58
Last Modified:11 Dec 2012 14:35

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