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H19 IS IMPRINTED IN THE CHOROID-PLEXUS AND LEPTOMENINGES OF THE MOUSE FETUS

Biomedical Sciences Research Institute Computer Science Research Institute Environmental Sciences Research Institute Nanotechnology & Advanced Materials Research Institute

SVENSSON, K, Walsh, Colum, FUNDELE, R and OHLSSON, R (1995) H19 IS IMPRINTED IN THE CHOROID-PLEXUS AND LEPTOMENINGES OF THE MOUSE FETUS. MECHANISMS OF DEVELOPMENT, 51 (1). pp. 31-37. [Journal article]

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DOI: 10.1016/0925-4773(94)00345-N

Abstract

It has been proposed that either the Igf-2 gene or the H19 gene - but not both - can be expressed from a given chromosome. Igf-2 is known to be biallelically expressed in the choroid plexus and leptomeninges, however, raising the question of whether H19 is down-regulated or absent there. We found by in situ hybridization that H19 is indeed expressed in the choroid plexus and leptomeninges of the developing mouse foetus. Comparison with the expression pattern of Igf-2 showed that the genes are coexpressed in all areas, with the exception of the choroid plexus epithelium. To evaluate whether H19 is also biallelically expressed in these tissues, we microdissected embryos from interspecific crosses and performed RNAse protection analysis on the isolated RNA. This revealed that H19 maintains its imprint in the choroid plexus/leptomeninges, being transcribed from the maternal allele at a level comparable to that in normal liver. We discuss the significance of these results for current models of Igf-2 and H19 imprinting.

Item Type:Journal article
Faculties and Schools:Faculty of Life and Health Sciences
Faculty of Life and Health Sciences > School of Biomedical Sciences
Research Institutes and Groups:Biomedical Sciences Research Institute
Biomedical Sciences Research Institute > Molecular Medicine
Biomedical Sciences Research Institute > Molecular Medicine > Transcriptional Regulation & Epigenetics
ID Code:21700
Deposited By:Professor Colum Walsh
Deposited On:02 Apr 2012 11:04
Last Modified:11 Dec 2012 14:52

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